Photo of Marna Yandeau-Nelson

Marna D. Yandeau-Nelson

Position
  • Associate Professor

Contact Info

2210 Molecular Biology Building, 2437 Pammel Drive
Ames
,
IA
50011-1079
Email
myn@iastate.edu
Phone
515-294-1079
Social Media and Websites

Education

  • B.S., Biology, Drake University, 1998
  • Ph.D., Genetics, Iowa State University, 2005

More Information

Dr. Yandeau-Nelson received her B.S. in Biology from Drake University in 1998 and her Ph.D. in Genetics in 2005 from Iowa State University. Her graduate thesis work characterized meiotic recombination in maize. As a postdoctoral scholar at Penn State University from 2005-2008, her work focused on the genetics of starch biosynthesis in maize.  In 2009, she returned to Iowa State University as an Associate Scientist and Graduate Faculty in the Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, and the NSF-Engineering Research Center for Biorenewable Chemicals (CBiRC).  She joined the faculty of the Genetics, Development, and Cell Biology Department in 2014.

Research Description

My research interests integrate classical and molecular genetics with biochemical, metabolomic and computational approaches to study the biosynthetic and regulatory genetic networks of metabolic traits, to increase both the fundamental knowledge of metabolism and to use that knowledge for practical applications.

I. Understanding the genetic network of plant cuticle lipids and their protective properties against environmental stresses: My group is using the stigmatic silks of maize as the model system to dissect the genetic and metabolic networks responsible for the synthesis of plant cuticle lipids, which are derived from fatty acids and accumulate on the aerial surfaces of plants. We are using the cuticle lipid metabolome as the model to study how the organism adapts and protects plant surfaces from environmental stresses. This work aims to reveal unique mechanisms that produce the distinctive surface chemistries that plants utilize to gain protection from environmental stresses. Based on the chemical similarity of surface lipid constituents to components of petroleum, this work has potential applications in bioengineering these networks to produce advance biofuels in heterologous systems (e.g. algae). Project website

II. Harnessing natural metabolic pathways for the production of biorenewable compounds: The ISU-based, NSF-funded Center for Biorenewable Chemicals (CBiRC) is leading the emerging bio-based chemical field by developing a flexible platform that combines biological and chemical catalysis to produce precursors for chemicals. Within the context of CBiRC, we are addressing how the fatty acid biosynthetic pathway can be harnessed in genetically tractable microbes to produce a variety of precursors for the emerging biorenewable chemicals industry. In a broader context, we are employing forward genetic approaches to study the regulation of fatty acid synthesis, and we are particularly interested in the “non-obvious” genetic determinants, and variants thereof, that determine fatty acid production.

Publications

  • Alexander LE, Okazaki Y, Schelling MA, Davis A, Zheng X, Rizhsky L, Yandeau-Nelson M.D., Saito K, Nikolau BJ (2020) Maize Glossy2 and Glossy2-like genes have overlapping and distinct functions in cuticular lipid deposition. Plant Physiol 183: 840-853 doi: 10.1104/pp.20.00241

  • Dennison T.S., Qin W., Loneman D.M., Condon S.G.F., Lauter N., Nikolau B.J., Yandeau-Nelson M.D. (2019) Genetic and environmental variation impact the cuticular hydrocarbon metabolome on the stigmatic surfaces of maize.  BMC Plant Biol 19:430  doi: 10.1186/s12870-019-2040-3

  • Sofeo N, Hart JH, Butler B, Oliver DJ, Yandeau-Nelson M.D., Nikolau BJ. (2019) Altering the substrate specificity  of acetyl-CoA synthetase by rational mutagenesis of the carboxylate binding pocket. ACS Synthetic Biology. DOI: 10.1021/acssynbio.9b00008

  • Lopez MD, Dennison TS, Paque TM, Yandeau-Nelson M.D., Abel CA, Lauter N. (2019) Development and application of a quantitative bioassay to evaluate maize silk resistance to corn earworm herbivory among progenies derived from Peruvian landrace Piura. PLoS ONE  14(4): e0215414. doi: 10.1371/journal.pone.0215414    

  • Jing, F., Yandeau-Nelson, M.D.*, Nikolau, B.J.* (2018) Identification of active site residues implies a two-step catalytic mechanism for acyl-ACP thioesterase. Biochemical Journal 475: 386103873 doi: 10.1042/BCJ20180470 *Co-corresponding authors

  • Jing, F., Zhao, L., Yandeau-Nelson, M.D., Nikolau, B.J. (2018) Dissecting the structural determinants of the substrate chain length selectivity of plant acyl-ACP thioesterases. Nature Commun, 9: 860 doi:10.1038/s41467-018-03310-z

  • Loneman, D.*, Peddicord, L.*, Al-Rashid, A., Nikolau, B.J., Lauter, N., Yandeau-Nelson, M.D. (2017) A robust and efficient method for the extraction of plant extracellular surface lipids as applied to the analysis of silks and seedling leaves of maize. PLOS ONE 12(7): e0180850 doi: 10.1371/journal.pone.0180850. *Contributed equally to this work.

  • Feenstra, A., Alexander, L., Song, Z., Korte, A., Yandeau-Nelson, M.D., Nikolau, B.J., Lee, Y-J. (2017) Spatial Mapping and profiling of metabolite distributions during germination of maize seeds. Plant Physiol, doi:10.1104/pp.17.00652

  • Duenas, M., Klein, A., Alexander, L., Yandeau-Nelson, M.D., Nikolau, B.J., Lee, Y-J. (2017) High-spatial resolution mass spectrometry imaging reveals the genetically programmed, developmental modification of the distribution of thylakoid membrane lipids among individual cells of the maize leaf. Plant J, 89: 825-838 doi:10.1111/tpj.13422

  • Garg, S., Rizhsky, L., Jin, H., Yu, X., Jing, F., Yandeau-Nelson, M.D., Nikolau, B.J. (2016) Microbial production of bi-functional molecules by diversification of the fatty acid pathway. Metabolic Engineering. 35: 9-20.

  • Yandeau-Nelson, M.D.*, Lauter, N., Zabotina, O.A. (2015) Advances in Metabolomic applications in plant genetics and breeding. CAB Reviews 10: 040 doi: 10.1079/PAVSNNR201510040
  *Corresponding author.

  • Korte, A., Yandeau-Nelson, M.D., Nikolau, B.J., Lee, Y-J. (2015) Sub-cellular Level Resolution MALDI-MS Imaging of Maize Leaf Metabolites by MALDI-Linear Ion Trap- Orbitrap Mass Spectrometer. Anal Bioanal Chem 407: 2301-2309 PMID 25618761.

  • Muszynski, M.G., and Yandeau-Nelson, M.D. (2014) Molecular Genetics of Bioenergy Traits. In: Goldman SL & Kole C (eds) Compendium of Bioenergy Plants: Corn. CRC Press, 169-197.

  • Jing, F., Cantu, D.C., Tvaruzkova, J., Chipman, J., Nikolau, B.J., Yandeau-Nelson, M.D., Reilly, P.J. (2011) Phylogenetic and experimental characterization of an acyl-ACP thioesterase family reveals significant diversity in enzymatic specificity and activity. BMC Biochem 12:44 doi:10.1186/1471-2091-12-44

  • Xia, H., Yandeau-Nelson, M., Thompson, D.B., Guiltinan, M.J. (2011) Deficiency of Maize Starch-Branching Enzyme I Results in Altered Starch Fine Structure, Decreased Digestibility and Reduced Coleoptile Growth During Germination. BMC Plant Biology, 11: 95.

  • Yandeau-Nelson, M.D., Laurens, L., Shi, Z., Xia, H., Smith, A.M., Guiltinan, M.J. (2011) Starch Branching Enzyme IIa is required for proper diurnal cycling of starch in leaves of Zea mays. Plant Physiol, doi: 10.1104/pp.111.174094

  • Perera, M.A., Qin, W., Yandeau-Nelson, M., Fan, L., Dixon, P., Nikolau, B.J. (2010) Biological origins of normal-chain hydrocarbons: a pathway model based on cuticular wax analyses of maize silks. Plant J, 64: 618-632.

  • Emrich, S.J., Li, L., Wen, T-J., Yandeau-Nelson, M.D., Fu, Y., Guo, L., Chou, H-H., Aluru, S., Ashlock, D.A., Schnable, P.S. (2007) Nearly identical paralogs (NIPs): implications for maize (Zea mays L.) genome evolution. Genetics, 175: 429-439.

  • Yandeau-Nelson, M.D., Nikolau, B.J., Schnable, P.S. (2006) Effects of trans-acting genetic modifiers on meiotic recombination across the a1-sh2 interval of maize. Genetics, 174: 101-112.

  • Yandeau-Nelson, M.D., Xia, Y., Li, J., Neuffer, M.G., Schnable, P.S. (2006) Unequal sister chromatid and homolog recombination at a tandem duplication of the a1 locus in maize. Genetics, 173: 2211-2226.

  • Hsia, A-P., Wen, T-J., Chen, H.D., Liu, Z., Yandeau-Nelson, M.D., Wei, Y., Guo, L., Schnable, P.S. (2005) Temperature gradient capillary electrophoresis (TGCE) – a tool for the high-throughput discovery and mapping of SNPs and IDPs. Theoretical and Applied Genetics, 111: 218-225.

  • Dietrich, C., Perera, M.A., Yandeau-Nelson, M.D., Meeley, R., Nikolau, B., Schnable, P. (2005) Characterization of two gl8 paralogs reveals that the 3-ketoacyl reductase component of fatty acid elongase is essential for maize (Zea mays L.) development. Plant Journal, 42: 844-861.

  • Yandeau-Nelson, M.D.*, Zhou, Q.Z.*, Yao, H.*, Xu, X.*, Nikolau, B.J., Schnable, P.S. (2005) MuDR transposase increases the frequency of meiotic crossovers in the vicinity of a Mu insertion in the maize a1 gene. Genetics, 169: 917-929. * Each of these authors contributed substantially to this work.

  • Skibbe, D.S., Liu, F., Wen, T.J., Yandeau, M.D., Cui, X., Cao, J., Simmons, C.R., Schnable, P.S. (2002) Characterization of the aldehyde dehydrogenase gene families of Zea mays and Arabidopsis. Plant Molecular Biology, 48:751-764.

  • Yao, H., Zhou, Q., Li, J., Smith, H., Yandeau, M., Nikolau, B.J., Schnable, P.S. (2002) Molecular characterization of meiotic recombination across the 140-kb multigenic a1-sh2 interval of maize. Proceedings of the National Academy of Sciences (USA), 99: 6157-6162.