GDCB Seminar — 'Phase transitioning ARF transcription factors to regulate hormone response'

Tuesday, February 26, 2019 - 4:10pm to 5:00pm
Event Type: 

Lucia Strader

Speaker — Lucia Strader, Associate Professor of Biology and the Center for Engineering MechanoBiology at Washington University in St. Louis

Title —  Phase transitioning ARF transcription factors to regulate hormone response

Abstract — The phytohormone auxin plays crucial roles in nearly every aspect of plant growth and development. The AUXIN RESPONSE FACTOR (ARF) family of transcription factors regulates auxin-responsive gene expression and exhibit nuclear localization in regions of high auxin responsiveness. Here we show that the ARF7 and ARF19 proteins accumulate in micron-sized assemblies within the cytoplasm of tissues with attenuated auxin responsiveness. We found that the intrinsically disordered middle region and the folded PB1 interaction domain of ARFs drive protein assembly formation. Mutation of a single lysine within the PB1 domain abrogates cytoplasmic assemblies, promotes ARF nuclear localization, and results in an altered transcriptome and morphological defects. Our data suggest a model in which ARF nucleo-cytoplasmic partitioning regulates auxin responsiveness, thus providing a mechanism for cellular competence for auxin signaling.

Host — Dior Kelley