'Mechanisms of rRNA Recycling in Eukaryotes and What Happens When They Fail'

Thursday, October 12, 2017 - 4:10pm
Event Type: 

Photo of Gustavo MacIntoshSpeaker: Dr. Gustavo MacIntosh, Associate Professor in Iowa State University Department of Biochemistry, Biophysics and Molecular Biology

Title: Mechanisms of rRNA Recycling in Eukaryotes and What Happens When They Fail

Abstract: Ribosomes are major components of all cells. Much is understood about their biosynthesis and several pathways have been characterized for the elimination of misassembled ribosomal subunits. However, little is known about the decay of stable ribosomes and recycling of rRNA.

RNase T2 ribonucleases represent one of the most widely distributed RNase families, and they are conserved in all eukaryotes, many prokaryotes, and some viruses. Our work showed that RNS2, a class II RNase T2 in Arabidopsis thaliana, is essential for normal ribosomal RNA decay. Null rns2-2 plants show constitutive autophagy, RNA accumulation in the vacuole, and longer rRNA half-life. Autophagosomes in rns2-2 mutants contain RNA and ribosomes, suggesting that autophagy is activated as an attempt to compensate for loss of rRNA degradation. Total RNA levels increase in rns2-2, in the autophagy mutants atg9-4 and atg5-1, which are deficient in core autophagy machinery, and in rns2-2atg9-4 and rns2-2atg5-1 double mutants. These results suggest that, together with RNS2, autophagy has a role in RNA turnover. In addition, rRNA accumulates in the vacuole in rns2-2 mutants. Vacuolar accumulation of rRNA was blocked by disrupting autophagy via an rns2-2atg5-1 double mutant but not by an rns2-2atg9-4 double mutant. Furthermore, atg5-1 vacuoles had significantly lower amounts of rRNA than WT or atg9-4. Thus, it is clear that ATG5 and ATG9 have different roles in the turnover of rRNA.

Our results suggest that an ATG5-dependet selective autophagic process transport rRNA to the vacuole for degradation by RNS2’ and disruption of this process in the rns2-2 mutant triggers general macroautophagy, which depends on ATG9 and ATG5.

Host: BBMB Department Chair Kristen Johansen

Refreshments will be served at 3:45 p.m. in the Molecular Biology Building Atrium.