Dr. McGrail, Associate Professor, received her BS in Biochemistry from the University of Massachusetts, Amherst and her PhD in Molecular and Cellular Biology from the University of Minnesota. Her previous positions include postdoctoral work at the University of California-San Diego, Myriad Genetics in Salt Lake City, and the Huntsman Cancer Institute at the University of Utah.
The McGrail lab studies brain development and disease using the zebrafish genetic model system. We develop CRISPR gene editing tools for functional genomics and disease modeling. Our research examines RB and TP53 tumor suppressors in brain development, in order to understand how their loss drives brain tumor cell proliferation and survival. We are also building novel in vivo models of neural inflammation using CRISPR to investigate pathways driving neurodegenerative disease.
Complete Publication List
Almeida MP, Kambakam S, Fang L, Ming Z, Welker JM, Schultz-Rogers LE, Ekker SC, Clark KJ, Essner JJ, McGrail M. Zebrafish Cre/lox regulated UFlip alleles generated by CRISPR/Cas targeted integration provide cell-type specific conditional gene inactivation. bioRxiv 2021.
Almeida MP, Welker JM, Siddiqui S, Luiken J, Ekker SC, Clark KJ, Essner JJ, McGrail M. Endogenous zebrafish proneural Cre drivers generated by CRISPR/Cas9 short homology directed targeted integration. Sci Rep. 2021 Jan 18;11(1):1732. doi: 10.1038/s41598-021-81239-y.
Welker JM, Wierson WA, Almeida MP, Mann CM, Torrie ME, Ming Z, Ekker SC, Clark KJ, Dobbs DL, Essner JJ, McGrail M. GeneWeld: a method for efficient targeted integration directed by short homology in zebrafish. Bio-protocol 2021 July 21; 11(14): e4100. DOI: 10.21769/BioProtoc.4100.
Wierson WA, Welker JM, Almeida MP, Mann CM, Webster DA, Weiss TJ, Torrie ME, Volbrecht MK, Lan M, McKeighan KC, Leavy J, Ming Z, Wehmeier A, Mikelson CS, Haltom JA, Kwan KM, Chien C-B, Balciunas D, Ekker SC, Clark KJ, Webber BR, Moriarity BS, Solin SL, Carlson DF, Dobbs DL, McGrail M*, Essner JJ*. Efficient targeted integration directed by short homology in zebrafish and mammalian cells. eLife. 2020 May 15;9. pii: e53968. doi: 10.7554/eLife.53968. PMID:32412410 * co-corresponding authors.
Ichino N, Serres M, Urban R, Urban M, Schaefbauer K, Greif L, Varshney GK, Skuster KJ, McNulty M, Daby C, Wang Y, Liao H-k, El-Rass S, Ding Y, Liu W, Schimmenti LA, Sivasubbu S, Balciunas D, Hammerschmidt M, Farber SA, Wen X-Y, Xu X, McGrail M, Essner JJ, Burgess S, Clark KJ, Ekker SC. Building the vertebrate codex using the Gene Breaking Protein Trap Library. eLife 2020; 9:e54572 DOI: 10.7554/eLife.54572
Wierson WA, Simone BW, WareJoncas Z, Mann C, Welker JM, Kar B, Emch MJ, Friedberg I, Gendron WAC, Barry MA, Clark KJ, Dobbs DL, McGrail MA, Ekker SC, Essner JJ. Expanding the CRISPR Toolbox with ErCas12a in Zebrafish and Human Cells. CRISPR J. 2019 Nov 19. doi: 10.1089/crispr.2019.0026. PMCID: PMC6919245.
Mann CM, Martínez-Gálvez G, Welker JM, Wierson WA, Ata H, Almeida MP, Clark KJ, Essner JJ, McGrail M, Ekker SC, Dobbs D. The Gene Sculpt Suite: a set of tools for genome editing. Nucleic Acids Res. 2019 Jul 2;47(W1):W175-W182. doi: 10.1093/nar/gkz405. PMCID: PMC6602503.
Schultz LE, Almeida MP, Wierson WA, Kool M, McGrail M. Retinoblastoma binding protein 4 maintains cycling neural stem cells and prevents DNA damage and Tp53-dependent apoptosis in neural progenitors. bioRxiv doi: http://dx.doi.org/10.1101/427344.
Schultz LE, Haltom JA, Almeida MP, Wierson WA, Solin SL, Weiss TJ, Helmer JA, Sandquist EJ, Shive HR, McGrail M. Epigenetic regulators Rbbp4 and Hdac1 are overexpressed in zebrafish RB-embryonal brain tumors and required for neural progenitor survival and proliferation. Disease Models & Mechanisms. 2018 Jun 15;11(6). pii: dmm034124. doi: 10.1242/dmm.034124. PMCID: PMC6031359.
Schultz LE, Solin SL, Wierson WA, Lovan JM, Syrkin-Nikolau J, Lincow DE, Severin AJ, Sakaguchi DS, McGrail M. VEGFA and Leptin expression associated with ectopic proliferation and retinal dysplasia in zebrafish optic pathway tumors. Zebrafish. 2017 Aug;14(4):343-356. doi: 10.1089/zeb.2016.1366.
Welker JM, Wierson WA, Wang Y, Poshusta TL, McNulty MS, Tisdale EE, Solin SL, Ekker SC, Clark KJ, McGrail MA, Essner JJ. GoldyTALEN vectors with improved efficiency for Golden Gate TALEN Assembly. Human Gene Therapy. 2016 10.1089/hum.2016.012.
Solin SL, Shive HR, Woolard KR, Essner JJ, McGrail M. Rapid tumor induction in zebrafish by TALEN-mediated somatic inactivation of the zebrafish retinoblastoma1 tumor suppressor rb1. Scientific Reports 2015 5, 13745; doi: 10.1038/srep13745.