Yanhai Yin

People
Professor
Yanhai Yin

Research Description

My laboratory is interested in understanding the molecular mechanisms and gene regulatory networks  through which plant steroid hormone, Brassinosteroid (BRs), regulate plant growth and stress responses. We use a combination of genetics, genomics, computational modeling and predicative phenomics approaches and model plant Arabidopsis thaliana in our research. The long-term goal is to apply the knowledge generated from model systems to improve crop production under adverse climate conditions. There are several lines of research:

  1. Investigate the Mechanisms and Networks for BR Regulated Plant Growth and Stress Responses: We have previously established that BRs function through BES1/BZR1 family transcriptional factors to mediate BR responses. We have identified many BES1 interacting proteins and target genes, based on which we have established BR Gene Regulatory Networks (GRNs) by computational modeling, in collaboration with M. Aluru and S. Aluru at Georgia Tech. We are currently investigating the functions of genes in the BR regulatory networks and the mechanisms of actions in plant growth and stress responses.
  2. Use Predicative Phenomics to Study BR Gene Regulatory Networks (GRNs): In collaboration with several groups including L. Tang, P. Schnable, S. Sarkar, L. Dong and S. Howell, we are developing new phenotyping tools that can be used to study the functions of a large number of genes in GRNs. These include developing a platform to phenotype plant growth under drought conditions and using the platform to study the functions of hundreds of genes identified by computational modeling and quantitative genetics. The goal is to establish relationship between GRNs generated from predicative modeling and plant performance under various stress conditions.
  3. Define the Functions and Signaling Pathways for the HERCULES Receptor-Like Kinases (HERKs): We have discovered that several CrRLK1L family receptor kinase genes, including HERK1, THE1 (THESEUS 1) and FER (FERONIA), are regulated by BRs and involved in plant growth and immunity.  We are using multidisciplinary approaches to study signaling transduction pathways for these receptor kinases. Our collaborators on this project include Y. Lee, E. Yu laboratories.

Publications

  • Nolan, T.M., Brennan, B., Yang, M., Chen, J., Zhang, M.,  Li, Z., Wang, X., Bassham, D., Walley, J. and Yin Y*. 2017 Selective Autophagy of BES1 Mediated by DSK2 Balances Plant Growth and Survival. Dev. Cell.  41: 33-46.
  • Ye, H., Liu, S., Tang, B., Chen, J., Xie, Z., Nolan, T.M., Jiang, H., Guo, H., Lin, H.-Y., Li, L., Wang, Y., Tong, H., Zhang, M., Li, Z., Chu, C., Aluru, M., Aluru, S., Schnable, P. S. S., Yin, Y*. 2017. RD26 mediates crosstalk between drought and brassinosteroid signaling pathway. Nature Communications. 8: 14753.
  • Chen, J., Nolan, T.M., Ye, H., Zhang, M., Tong, H., Xin, P., Chu, J., Chu, C., Li, Z., Yin, Y*. 2017. Arabidopsis WRKY46, WRKY54 and WRKY70 Transcription Factors Are Involved in Brassinosteroid-Regulated Plant Growth and Drought Response. The Plant Cell. 29: 1425-1439.
  • Nolan, T.M., Chen, J. and Yin, Y*. 2017. Cross-talk of Brassinosteroid Signaling in Controlling Growth and Stress Responses. Biochem. J. 474: 2641-2661.
  • Yang, M., Li, C., Cai, Z., Hu, Y., Nolan, T., Yu, F., Yin, Y., Xie, Q., Tang, G., Wang, X. 2017. SINAT E3 ligases control the light-mediated stability of the brassinosteroid-activated transcription factor BES1 in Arabidopsis. Dev. Cell. 41: 47-58.
  • Nolan, T., Liu, S., Guo, H., Li, L., Schnable, S. & Yin, Y*. (2017). Identification of Brassinosteroid Target Genes by Chromatin Immunoprecipitation Followed by High­throughput Sequencing (ChIP­seq) and RNA­seq. Method in Molecular Biology. 1564: 63-79.
  • Deng, X.G., Zhu, T., Peng, X.J., Xi, D.H., Guo, H., Yin, Y., Zhang D.W., Lin, H.H. 2016. Role of brassinosteroid signaling in modulating Tobacco mosaic virus resistance in Nicotiana benthamiana. Sci Rep. 2016 Feb 3; 6: 20579.
  • Kir, G., Ye, H., Nelissen, H., Neelakandan, A.K., Kusnandar, A.S., Luo, A., Inze, D., Sylvester, A., Yin, Y., Becraft, P.W. (2015) RNAi knockdown of BRI1 in maize (Zea mays) reveals novel functions for brassinosteroid signaling in controlling plant architecture. Plant Physiol. 169: 826-839.
  • Wang, X., Chen J., Xie, Z., Liu, S., Nolan, T., Ye, H., Zhang, M., Guo, H., Schnable, P.S., Li, Z. and Yin, Y. 2014. Histone Lysine Methyltransferase SDG8 Is Involved in Brassinosteroid Regulated Gene Expression in Arabidopsis thaliana. Molecular Plant. 7: 1303-1315.
  • Tong, H., Xiao, Y., Liu, D., Gao, S., Liu, L., Yin, Y., Jin, Y. Qian, Q., and Chu, C. 2014. Brassinosteroid regulates cell elongation by modulating gibberellin metabolism in rice. Plant Cell, 26: 4376-93.
  • Mantilla Perez, M.B., Zhao, J., Yin, Y., Hu, J., Salas Fernandez, M.G. 2014. Association mapping of brassinosteroid candidate genes and plant architecture in a diverse panel of Sorghum bicolor. Theor Appl Genet. 127: 2645-62.
  • Zhang, D., Yuan, S., Xu, F., Zhu, F., Yuan, M., Ye, H., Guo, H., Lv, X., Yin, Y. and Lin, H. 2014. Light intensity affects chlorophyll synthesis during greening process by metabolite signal from mitochondrial alternative oxidase in Arabidopsis. Plant Cell & Environment. 39: 12-25.
  • Zhang, D., Ye, H., Guo, H., Johnson, A., Zhang, M., Lin, H., and Yin, Y. 2014. Transcription Factor HAT1 is Phosphorylated by BIN2 Kinase and Mediates Brassinsteroid Repressed Gene Expression in Arabidopsis. Plant J. 77: 59-70
  • Zhang, D., Ye, H., Guo, H., Johnson, A., Zhang, M., Lin, H., and Yin, Y. 2014. Transcription factors involved in brassinosteroid repressed gene expression and their regulation by BIN2 kinase. Plant Signal Behav. 9: e27849
  • Guo H, Li L, Aluru M, Aluru S, Yin Y. 2013. Mechanisms and networks for brassinosteroid regulated gene expression. Curr Opin Plant Biol. 16:545-53.
  • Hao J. Yin, Y., Fei, S. 2013. Brassinosteroid Signaling network: implications on yield and stress tolerance.  Plant Cell Report. 32: 1017-1030.
  • Ye, H., Li, L., Guo, H., Yin Y. (2012) MYBL2 is a substrate of GSK3-like kinase BIN2 and acts as a corepressor of BES1 in brassinosteroid signaling pathway in Arabidopsis. Proc Natl Acad Sci U S A. 109: 20142-20147.
  • Tong, H., Liu, L., Jin, Y., Du, L., Yin Y., Qian, Q., Zhu, L. and Chu, C. 2012. DWARF AND LOW-TILLERING Acts as a Direct Downstream Target of a GSK3/SHAGGY-Like Kinase to Mediate Brassinosteroid Responses in Rice. Plant Cell24: 2562-2577.
  • Ye H, Li L, Yin Y (2011) Recent advances in the regulation of brassinosteroid signaling and biosynthesis pathways. J. Integr. Plant Biol. 53, 455–468.
  • Yu, X*, Li, L*, Zola, J, Aluru, M, Ye, H, Foudree, A, Guo, H, Anderson, S, Aluru, S, Liu, P, Rodermel, S, and Yin, Y. (2011) A Brassinosteroid transcriptional network revealed by genome-wide identification of BES1 target genes in Arabidopsis thaliana. Plant J. 65: 634-646. (*co-first authors).
  • Li, L., Yu, X., Ye, H., Guo, H., Yin, Y. (2010). Arabidopsis IWS1 interacts with transcription factor BES1 and is involved in Brassinosteroid regulated gene expression. Proc Natl Acad Sci U S A. 107: 3918-3923.
  • Ye, Q., Zhu, W., Li, L.,Yin, Y., Ma, H., Wang, X. 2010. Brassinosteriods control male fertility by regulating the expression of key genes involved in Arabidopsis anther and pollen development. Proc Natl Acad Sci USA 107: 6100-6105.
  • Guo, H., Li, L., Ye, H., Yu, X., Algreen, A.,Yin, Y. 2009. Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana. Proc Natl Acad Sci USA 106: 7648-7653.
  • Li, L., Yu, X., Thompson, A., Guo, M. Yoshida, S., Asami, T., Chory, J., Yin, Y. 2009. Arabidopsis MYB30 is a direct target of BES1 and cooperates with BES1 to regulate brassinosteroid target gene expression. Plant J. 58: 275-286.
  • Guo, H., Ye, H., Li, L.,Yin, Y. 2009. A Family of Receptor-Like Kinases Are Regulated by BES1 and Involved in Plant Growth in Arabidopsis thaliana. Plant Signaling & Behavior 4: 784-786.
  • Srivastava, R., Liu, J., Guo, H., Yin, Y., Howell, S. 2009. Regulation and processing of a plant peptide hormone, AtRALF23, in Arabidopsis. Plant J. 59: 930-939.
  • Tong, H., Jin, Y., Liu, W., Li, F., Yin Y., Qian, Q., Zhu, L. and Chu, C. 2009. Dwarf and low-tillering, a new member of the GRAS family, plays positive roles in brassinosteroid signaling in rice. Plant J. 58: 803-816.
  • Salas Fernandez, M.G., Becraft, P.W., Yin, Y., Lübberstedt, T. 2009. From dwarves to giants? Plant height manipulation for biomass yield.Trend Plant Sci 14: 454-461.
  • Yu, X., Li, L., Li, L., Guo, H., Chory, J., Yin, Y. 2008. Modulation of brassinosteroid-regulated gene expression by Jumonji domain-containing proteins ELF6 and REF6 in Arabidopsis. Proc Natl Acad Sci USA 105: 7618-7623.
  • Yin, Y. Vafeados, D., Tao, Y., Yoshida, S., Asami, T., Chory, J. 2005. A new class of transcription factors mediates brassinosteroid-regulated gene expression in Arabidopsis. Cell 120: 249-259.
  • Mora-Garcia, S., Vert, G., Yin, Y., Cano-Delgado, A., Cheong, H., and Chory, J. 2004. Nuclear protein phosphotases with Kelch-repeat domains modulate the response to bassinosteroids in Arabidopsis. Genes & Development 18: 448-460.
  • Caño-Delgado, A.*, Yin, Y.*, Yu, C.*, Vafeados, D., Mora-García, S., Cheng, J-C., Nam, K.H.2, Li, J. and Chory, J. 2004. BRL1 and BRL3 are novel brassinosteroid receptors that function in vascular differentiation in Arabidopsis. Development 131: 5341-5351 (*cofirst authors).
  • Yin, Y., Cheong, H., Friedrichsen, D., Zhao, Y., Hu, J., Mora-Garcia, S., and Chory, J. 2002. A crucial role for putative Arabidopsis topoisomerase VI in plant growth and development. Proc Natl Acad Sci USA 99: 10191-10196.
  • Yin, Y., Wang , Z-Y., Mora-Garcia, S., Li, J., Yoshida, S., Asami, T., and Chory, J. 2002. BES1 acccumulates in the nucleus in response to brassinosteroid to regulate gene expression and promotes stem elongation. Cell 109: 181-191.
  • Yin, Y., Zhu, Q., Dai, S., Lamb, C. and Beachy, R.N. 1997. RF2a, a bZIP transcriptional activator of the phloem-specific rice tungro bacilliform virus promoter, functions in vascular development. EMBO J. 16: 5247-5259.
Area of Expertise: 
Understanding how plant steroid hormones Brassinosteroids (BRs) regulate plant growth and development
Education: 
B.S., Biology, Sichuan University, Chendu, China, 1985
M.S., Cell Biology and Genetics, Institute of Botany, Chinese Academy of Sciences, Beijing, China, 1988
Ph.D., Macromolecular and Cellular Structure and Chemistry, The Scripps Research Institute, La Jolla, 1997
Contact
515-294-4816
1035D Roy J Carver Co-Lab
Ames
IA
50011-1085